Journal: Dentistry Journal

Article Title: In Vivo Evaluation of Regenerative Osteogenic Potential Using a Human Demineralized Dentin Matrix for Dental Application

doi: 10.3390/dj12030076

Figure Lengend Snippet: Representative histological images of (H&E) ( A , D , G , J ), Masson’s trichrome ( B , E , H , K ) and osteocalcin immune staining ( C , F , I , L ) are shown at 4 weeks post-treatment. Coronal sections through the mid-point of the defects were prepared from decalcified specimens. Fibrous connective tissues were seen to be mostly filled in the defect areas in the control group ( A – C ). The group treated with SA hydrogel as the only grafted material showing fibrous and adipose tissue ( D – F ). lacuna spaces were observed in the newly formed lamellar bone in NHH with a wide bone marrow space ( G – I ). DDMH-treated groups showed considerable new bone trabeculae formation ( J – L ). BT: bone trabeculae; FT: fibrous tissue. Arrows represent MT- and OCN-positive reactions. Scale bar: 100 μm.

Article Snippet: Subsequently, the samples were embedded in paraffin; and after staining with hematoxylin and eosin (H&E), Masson’s trichrome (MT) (Cat# HT15, Sigma Aldrich), and immunohistochemical staining with a polyclonal anti-rabbit osteocalcin antibody (OCN; Cat# GB11233, diagnostic biosystems) at a 1:150 dilution, histological examination was conducted using an Olympus BX-51 optical microscope (Olympus Co., Tokyo, Japan).

Techniques: Staining, Control

Journal: Dentistry Journal

Article Title: In Vivo Evaluation of Regenerative Osteogenic Potential Using a Human Demineralized Dentin Matrix for Dental Application

doi: 10.3390/dj12030076

Figure Lengend Snippet: Representative histological images of (H&E) ( A , D , G , J ), Masson’s trichrome ( B , E , H , K ) and osteocalcin immune staining ( C , F , I , L ) are shown at 8 weeks post-treatment. Rather than the newly created bone, the defect locations in the control group were found to be primarily filled with fibrous connective tissues and adipose tissue ( A – C ). Circular new bony islands with the presence of early woven bone were observed in the group treated with SA as the only grafted material ( D – F ). Newly formed lamellar bone was observed in NHH with a wide bone marrow space ( G – I ). In contrast, DDMH-treated groups showed considerable new bone formation at 8 weeks with relatively narrow marrow spaces ( J – L ). BT means bone trabeculae; FT: fibrous tissue. Arrows represent MT- and OCN-positive reactions. Scale bar: 100 μm.

Article Snippet: Subsequently, the samples were embedded in paraffin; and after staining with hematoxylin and eosin (H&E), Masson’s trichrome (MT) (Cat# HT15, Sigma Aldrich), and immunohistochemical staining with a polyclonal anti-rabbit osteocalcin antibody (OCN; Cat# GB11233, diagnostic biosystems) at a 1:150 dilution, histological examination was conducted using an Olympus BX-51 optical microscope (Olympus Co., Tokyo, Japan).

Techniques: Staining, Control

Journal: Bioactive Materials

Article Title: Throw out an oligopeptide to catch a protein: Deep learning and natural language processing-screened tripeptide PSP promotes Osteolectin-mediated vascularized bone regeneration

doi: 10.1016/j.bioactmat.2024.11.011

Figure Lengend Snippet: DL and NLP-screened tripeptide PSP promoted vascularized bone regeneration in critical-sized cranial defect model . (A) Illustration of the experimental procedure; (B) Representative SEM images of GelMA, PSP-GelMA and KLT-GelMA hydrogels (scale bar, 20 μm); Micro-CT reconstruction from top view, coronal view and sagittal view 4-weeks (C) and 8-weeks (D) after implantation (scale bar, 1 mm); (E) Semi-quantitative analysis of Micro-CT; Representative images of HE staining 4-weeks (F) and 8-weeks (G) after implantation (scale bar, low magnification 500 μm/high magnification 100 μm); Representative images of Masson's trichrome staining 4-weeks (H) and 8-weeks (I) after implantation (scale bar, low magnification 500 μm/high magnification 100 μm); Representative images of immunofluorescence staining of CD31 and EMCN 4-weeks (J) and 8-weeks (K) after implantation (DAPI in blue, CD31 in red, EMCN in green, scale bar, low magnification 500 μm/high magnification 50 μm, arrow indicated CD31 and EMCN co-labeled type H vessels). Data are presented as means ± SD. Significant difference with respect to the control groups (∗), ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: Primary mouse anti-CD31 antibody (1:200, 66065-2-lg, Proteintech, USA), primary rabbit anti-EMCN antibody (1:200, YT1557, Immunoway, USA) and primary rabbit anti-OCN antibody (1:200, GB11233, Servicebio, China) were used for immunofluorescence (IF) and immunohistochemistry (IHC) staining.

Techniques: Micro-CT, Staining, Immunofluorescence, Labeling, Control

Journal: Experimental & Molecular Medicine

Article Title: Local delivery of OSK factors enables partial cellular reprogramming to mitigate osteoarthritis and cartilage fibrosis

doi: 10.1038/s12276-026-01662-x

Figure Lengend Snippet: a RT–qPCR analysis of osteogenic genes ( Col1 , Runx2 and Osx ) and metabolic genes ( Acan , Col2 and Mmp13 ) in ATDC5 cells under osteogenic conditions for 3 days, after treatment with vehicle, AAV-Mock or AAV-OSK for 3 days. n = 3 per group. b , c ICC staining of SOX2 and RUNX2 in ATDC5 cells under osteogenic conditions ( b ) and the percentage of RUNX2-positive cells ( c ). n = 3 per group. Scale bar, 50 μm. d Immunofluorescence staining of RUNX2 in the joint of Col2α1-CreERT;Rosa26-tdTomato reporter mice following DMM surgery, treated with vehicle or AAV-OSK. Scale bar, 50 μm. e The percentage of RUNX2-positive cells in d . n = 3 per group. Data are presented as mean ± s.d. and analyzed using one-way ANOVA with Bonferroni post hoc test ( a and c ) or unpaired t -tests ( e ). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Primary antibodies against SOX2 (14-9811-82, Thermo Fisher), IL-1 (GB11113, Servicebio), RUNX2 (12556, CST), OSX (sc393325, Santa Cruz) and OCN (GB11233, Servicebio) were utilized.

Techniques: Quantitative RT-PCR, Staining, Immunofluorescence